Select the Appropriate Pipette: Choose a serological graduated pipette that suits your desired volume range. Ensure that the pipette's capacity exceeds the volume you need to measure or dispense.
Preparing the Pipette: Inspect the serological pipette for any damage or defects. If using disposable pipettes, open a new one. For reusable pipettes, ensure it is clean and dry. Attach a sterile pipette tip securely to the end for contamination prevention.
Setting the Volume: Some serological pipettes have adjustable volume settings. Locate the volume adjustment mechanism, usually a dial or plunger, and set it to the desired volume.
Aspiration: Immerse the serological pipette tip into the liquid solution at a slight angle. Depress the plunger or press the button to draw the liquid into the pipette. Make sure the liquid completely fills the pipette tip.
Dispensing: Carefully transfer the pipette to the destination vessel, such as a test tube or well plate. Align the pipette tip above the desired target and steadily depress the plunger to dispense the liquid. Ensure that the liquid completely exits the pipette tip without any residual droplets.
Disposal or Cleaning: If using disposable serological pipettes, immediately discard them in a designated waste container.