PakGent Bioscience (Suzhou) Co., Ltd.

PakGent 40ul qPCR 384-well plate

The 40ul 384-Well PCR Plate is a high-quality polypropylene PCR plate. It has an ultra-thin wall design and is also compatible with most thermal cyclers. Its clear bottom allows for better visualization of sample wells and its conical well design ensures even sample distribution. The 384 well plate qPCR has a uniform flat surface and is designed to prevent evaporation and cross contamination. Its flat-bottom wells maximize sample volume, and its anti-skid feature ensures plates stay in place during PCR cycling.

Parameter of 40ul qPCR plate 384

Part No.DescriptionPackageSize/CaseCBM/CaseGW/Case (kg)
PCRP-40FS-384C40ul 384-well, white base, clear wells10pcs/box, 10box/case47*29*14cm0.01912.63
PCRP-40FS-384C-W40ul 384-well, white base, white wells10pcs/box, 10box/case47*29*14cm0.01911.55

For more PakGent PCR consumables, inquire now!

Improving PCR Efficiency in 384-Well Plates qPCR

1. Use smaller reaction volumes for qPCR 384-well plate: Using smaller reaction volumes in 384-well plate qPCR can improve PCR efficiency by increasing the reaction surface area and reducing the amount of reagents used. This can also reduce the risk of contamination due to the smaller reaction volumes.

2. Minimize the distance between the reaction and the thermal cycler block: Increasing the distance between the reaction and the thermal cycler block can reduce the time needed to reach the desired temperature and therefore improve qPCR 384-well plate efficiency.

3. Use optimized reaction conditions for qPCR 384-well plate: Optimizing the reaction conditions, such as the amount of primer and template, the concentration of MgCl2 and the reaction temperature, can improve the PCR efficiency in 384-well plate qPCR.

4. Use pre-dispensed reagents for qPCR 384-well plate: Pre-dispensing the PCR reagents into the wells of the 384-well plate qPCR can reduce the risk of contamination and improve the efficiency of the PCR reaction.

5. Use high-quality reagents for qPCR 384-well plate: Using high-quality reagents can improve the efficiency of the PCR reaction by reducing the risk of contamination and ensuring that the reaction conditions are optimal.

6. Use improved thermal cyclers for qPCR 384-well plate: Using improved thermal cyclers with a faster ramping rate and a more accurate temperature control can improve the efficiency of the PCR reaction in 384-well plate qPCR.

Troubleshooting Guide for PCR Amplification Issues with the 40ul 384-Well PCR Plate

1. No amplification signal:

- Verify thermal cycler settings, PCR reagents, and proper sealing of the Pakgent qPCR 384-well plate.

- Adjust annealing temperature and extension time if needed.

2. Weak or low amplification signal:

- Check DNA quality and concentration.

- Optimize primer concentration and design.

- Increase the cycling cycles.

3. Non-specific amplification:

- Optimize annealing temperature and primer design.

- Consider touchdown or nested PCR protocols.

- Check for contamination in PCR reagents.

4. Noisy background or smearing:

- Ensure DNA template, primers, and PCR reagents are contamination-free.

- Optimize PCR conditions.

- Maintain a clean workspace.

5. Incomplete or partial amplification:

- Check DNA template quality and concentration.

- Verify primer specificity and adjust PCR conditions.

- Consider using more efficient DNA polymerase or enhancers.

If issues persist, consult technical support or the PakGent's troubleshooting guide.

Features and Benefits of the PakGent 40ul 384 qPCR Plate

The PakGent 40ul 384-Well PCR Plate is a high-quality labware designed for PCR applications. PakGent 384 well QPCR plate features include a durable polypropylene construction, accurate 40ul capacity per tube, compatibility with 384-well plate qPCR format, screw lids for easy access, uniform amplification, versatility for various PCR applications, compatibility with automated thermal cyclers, quality assurance, convenient storage, and cost-effectiveness in high-throughput workflows.

40ul 384-Well PCR Plate FAQs

What is the volume of a qPCR 384 well plate?

A qPCR 384-well plate typically has a well volume of 30-40 microliters. This 384 well plate volume range is ideal for conducting quantitative polymerase chain reaction (qPCR) experiments, providing a small but sufficient space for the reaction mixture. The small well size allows for high-throughput analysis of samples while conserving precious reagents. Additionally, the 384-well format is compatible with automated liquid handling systems, further streamlining the workflow for qPCR assays in research, diagnostics, and other molecular biology applications.

Can these PCR tubes be used for qPCR?

Yes, these 384-Well PCR Plate tubes can be used for quantitative PCR (qPCR) applications. Pakgent qPCR 384-well plates are designed to withstand the thermal cycling and fluorescence detection requirements of qPCR assays.

What material are these PCR tubes made from?

These PCR tubes are made from high-quality polypropylene, which is a durable and chemically resistant material commonly used in laboratory applications.

Are these PCR tubes compatible with automated thermal cyclers?

Yes, these PCR tubes are designed to be compatible with most automated thermal cyclers commonly used in laboratories, allowing for seamless integration into automated PCR workflows.

Can the PCR tubes be easily labeled for sample identification?

Yes, these pcr 384 well plate tubes usually have flat sides or frosted areas that allow for easy labeling using markers or labels, ensuring proper identification of samples during PCR experiments.

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