PakGent 96-Well Half Skirt, Fit Biorad/Roche PCR Plates

Parameter of 96-Well Half Skirt, Fit Biorad/Roche PCR Plates

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Biorad pcr plates Compatibility

Bio-Rad PCR plates are widely used in molecular biology research due to their high quality, reliability, and compatibility with a wide range of thermal cyclers. They are available in a variety of formats, including microplates, strips, and tubes, to suit different experimental needs.

Compatibility:

Bio-Rad PCR plates are designed to be compatible with most major thermal cyclers, including:

Bio-Rad CFX Real-Time PCR Systems

Bio-Rad T100 Thermal Cycler

Bio-Rad S1000 Thermal Cycler

Applied Biosystems StepOnePlus Real-Time PCR System

Roche LightCycler Real-Time PCR System

Thermo Fisher Scientific iQ5 Real-Time PCR System

To ensure compatibility, it is always recommended to check the specifications of your thermal cycler and the Bio-Rad PCR plates you are considering using.

Considerations for Choosing Bio-Rad PCR Plates

When choosing Bio-Rad PCR plates, it is important to consider the following factors:

Type of experiment: Different types of PCR experiments may have specific requirements for the plates. For example, real-time PCR (qPCR) experiments may require white reaction wells for better fluorescence detection, while standard PCR may use clear wells.

Volume requirements: Choose plates with the appropriate well volume capacity for your reaction needs. Bio-Rad PCR plates are available in a variety of well volumes, ranging from 96-well plates with 20 µL wells to 384-well plates with 5 µL wells.

Skirted or non-skirted: Skirted plates have a raised skirt around the wells that makes them easier to handle and stack, especially when working with automation systems. Non-skirted plates are generally more affordable but may be more difficult to handle manually.

Material: Bio-Rad PCR plates are typically made from polypropylene (PP) or polycarbonate (PC). PP is the most common material for PCR plates due to its inert properties and ability to withstand the thermal cycling conditions of PCR experiments. PC offers good clarity and can be used for qPCR applications where fluorescence needs to be measured. However, PC may not be compatible with all thermal cyclers due to its lower melting temperature compared to PP.

Sterility: Consider whether you need sterile plates to prevent contamination, especially for sensitive experiments. Bio-Rad offers both sterile and non-sterile PCR plates.