PakGent Bioscience (Suzhou) Co., Ltd.

PakGent Cell Culture Flask

Cell culture flasks are laboratory tools used to cultivate cells in a controlled environment. They come in different sizes and shapes and are typically made of plastic. The flasks have a flat bottom and feature a neck and cap. The neck is designed to allow for easy access to the cells, while the cap is used to maintain a sterile environment. The shape of the flask also allows for optimal gas exchange, which is important for cell growth. Additionally, cell culture flasks typically have a spout for easy pouring and disposal of media.

Parameter of Cell Culture Flask

Part No.DescriptionPackageSize/CaseCBM/CaseGW/Case (kg)
CL-F025P25cm2,Plug Cap, PS, sterile10/bag,20bags/case436*281*320mm0.03924.3
CL-FT025P25cm2, Plug Cap, PS, TC treated, sterile10/bag,20bags/case436*281*320mm0.03924.3
CL-F025V25cm2,Vent Cap, PS, sterile10/bag,20bags/case436*281*320mm0.03924.3
CL-FT025V25cm2, Vent Cap, PS, TC treated, sterile10/bag,20bags/case436*281*320mm0.03924.3
CL-F075P75cm2,Plug Cap, PS, sterile5/bag,20bags/case436*369*411mm0.06615.4
CL-FT075P75cm2, Plug Cap, PS, TC treated, sterile5/bag,20bags/case436*369*411mm0.06615.4
CL-F075V75cm2,Vent Cap, PS, sterile5/bag,20bags/case436*369*411mm0.06615.4
CL-FT075V75cm2, Vent Cap, PS, TC treated, sterile5/bag,20bags/case436*369*411mm0.06615.4
CL-F175P175cm2,Plug Cap, PS, sterile5/bag,6bags/case440*395*265mm0.04614.5
CL-FT175P175cm2, Plug Cap, PS, TC treated, sterile5/bag,6bags/case440*395*265mm0.04614.5
CL-F175V175cm2,Vent Cap, PS, sterile5/bag,6bags/case440*395*265mm0.04614.5
CL-FT175V175cm2, Vent Cap, PS, TC treated, sterile5/bag,6bags/case440*395*265mm0.04614.5
CL-F225PCell Culture Flask with Plug Cap, PS, 225cm25/pack, 5bags/case810*310*300mm0.07535.76
CL-FT225P225cm2, Plug Cap, PS, TC treated, sterile5/bag,5bags/case810*310*300mm0.07535.76
CL-F225V225cm2,Vent Cap, PS, sterile5/bag,5bags/case810*310*300mm0.07535.76
CL-FT225V225cm2, Vent Cap, PS, TC treated, sterile5/bag,5bags/case810*310*300mm0.07535.76

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PakGent Cell Culture Flask Sizes and their uses

PakGent offers a range of cell culture flask sizes tailored to various research and laboratory needs. The T25 cell culture flask, with a working volume of 25 cm², is ideal for small-scale experiments and initial cell growth. It's commonly used for seeding and maintaining adherent cell lines.

Stepping up in size, the T75 cell culture flask provides a larger surface area of 75 cm², accommodating higher cell densities and facilitating increased cell proliferation. This size is frequently employed for routine cell culture tasks and medium-scale experiments.

For larger-scale applications, the T175 cell culture flask offers a generous surface area of 175 cm², suitable for bulk cell culture expansion, protein expression, and other high-volume assays. Its increased capacity allows for greater cell yield without frequent passaging.

At the top end of the spectrum, the T225 cell culture flask boasts an expansive surface area of 225 cm², catering to extensive cell culture needs such as large-scale production, viral propagation, and bioprocessing. Its spacious design minimizes the need for frequent media changes and passaging, optimizing efficiency in demanding research settings. Whether for small-scale studies or large-scale production, PakGent's diverse flask sizes ensure flexibility and reliability in cell culture applications.

PakGent tissue culture flask coating and material selections

Selecting the appropriate material and coating for tissue culture flasks is pivotal in ensuring successful cell culture experiments. The material of the flask, such as polystyrene (PS), polymethylpentene (PMP), or treated polystyrene (TC-PS), profoundly impacts cell behavior. PS, the most prevalent choice, is cost-effective but may absorb proteins from the medium, affecting cell attachment. PMP offers enhanced gas permeability and reduced protein absorption compared to PS. TC-PS, treated to improve cell attachment, is widely preferred in cell culture applications.

Coating the flask surface further influences cell adhesion, growth, and differentiation. Coatings like extracellular matrix (ECM) proteins (e.g., collagen, laminin), synthetic polymers (e.g., poly-L-lysine, polyethyleneimine), and basement membrane matrix (BME) mimic natural cellular environments and facilitate specific cell interactions.

Choosing the optimal combination of material and coating depends on several factors, including cell type (adherent or suspension), cell line requirements, and experimental objectives. Adherent cells necessitate surfaces promoting attachment, while suspension cells may not. Additionally, experimental goals, such as proliferation or differentiation studies, inform the selection process. By carefully considering these factors, researchers can optimize flask material and coating choices for their specific cell culture needs, fostering successful outcomes in their experiments.

Cell Culture Flask FAQs

What is the best way to store a Cell Culture Flask?

Cell culture flasks should be stored in a cool, dry place away from direct sunlight. Additionally, it is important to keep the cap on the flask to prevent contamination.

How often should a Cell Culture Flask be changed?

A cell culture flask should be changed every 3-4 days or when the cells start to overgrow. It is important to change the flask to prevent the cells from becoming overcrowded or contaminated.

What are the safety precautions that should be taken when working with Cell Culture Flasks?

When working with cell culture flasks, it is important to take safety precautions such as wearing gloves, using sterile pipettes, and disinfecting surfaces. Additionally, it is important to practice good lab etiquette, such as labeling flasks and disposing of them properly.

How do you dispose of a Cell Culture Flask?

Cell culture flasks should be autoclaved before disposal. After autoclaving, the flasks should be placed in a designated biohazard waste container for proper disposal.

What are the benefits of using a Cell Culture Flask?

The benefits of using a cell culture flask include the ability to provide an optimal environment for cell growth, the ability to monitor cell growth and behavior, and the ability to perform experiments with cells. Additionally, cell culture flasks are durable and can be reused multiple times.

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