Our tube design and thickness offers the perfect seal, since the seal between the tube opening and the tube cap is critical in minimizing evaporation or sample leakage. Loose caps allow evaporation; overly tight caps cause spillage upon opening. All PCR tubes are non-sterile, certified RNase and DNase free.
Available in a wide variety of styles from individual tubes, to strips and plates.
Most of PCR plates have both clear and white type
Numeric markings on our strip of tubes
Alphaumeric markings on plates
PCR consumables are disposable plastic items used in polymerase chain reaction (PCR) experiments. These consumables play a vital role in ensuring the success of your PCR as they can influence factors like contamination, sensitivity, and overall efficiency.
Common materials for PCR consumables:
Polypropylene (PP):This is the most widely used material for PCR tubes and plates due to its inert properties and ability to withstand the thermal cycling conditions of PCR experiments.
Polycarbonate (PC):This material offers good clarity and can be used for real-time PCR (qPCR) applications where fluorescence needs to be measured. However, PC may not be compatible with all thermal cyclers due to its lower melting temperature compared to PP.
Skirted PCR tubes and plates: These consumables have a skirt around the base that allows for better handling during automation and robotic liquid handling systems.
Choosing the right PCR consumables:
Here are some key factors to consider when choosing PCR consumables:
Type of PCR experiment: Different types of PCR experiments may have specific requirements for the consumables. For example, qPCR experiments may require white reaction tubes for better fluorescence detection, while standard PCR may use clear tubes.
Compatibility with your thermal cycler: Ensure the tubes or plates you choose are compatible with the thermal cycler you are using. This includes factors like size, material, and skirt design.
Volume requirements: Choose tubes or plates with the appropriate volume capacity for your reaction needs.
Sterility: Consider whether you need sterile consumables to prevent contamination, especially for sensitive experiments.
Cost: PCR consumables are available from a variety of manufacturers at different price points. Consider the balance between cost and quality for your specific needs.
By considering these factors, you can choose the most appropriate PCR consumables for your experiments and ensure optimal results.
PCR consumables are used in a variety of diagnostic applications. PakGent 384 well plate qPCR and PCR sealer and other PCR consumables, They allow for the rapid and accurate detection of infectious diseases, genetic diseases, and other abnormalities in a sample. They can also be used to detect minuscule amounts of DNA present in the sample, such as in forensic investigations.
PCR can also be used to create rapid, multiplexed tests that can be used to detect multiple diseases in a single assay. Additionally, PCR can be used to compare genetic sequences in order to perform genotyping, which can be used for disease diagnosis and prognosis. PCR consumables are also used in the research environment to produce large amounts of DNA for further characterization.
Yes, PCR consumables are designed to be compatible with a wide range of PCR instruments. It is important to ensure that the consumables you choose match the specifications and requirements of your specific PCR instrument.
Most PCR plastic consumables, such as tubes, plates, and strip tubes, are supplied as sterile products to prevent contamination. However, it is always recommended to double-check the packaging or product description to confirm the sterility of the consumables before use.
PCR consumables like tubes and plates are typically designed for single-use only. Reusing them can increase the risk of contamination and affect the accuracy of the results. It is recommended to use fresh, sterile consumables for each PCR experiment.
It is important to handle PCR consumables with clean gloves or pipette tips to avoid contamination. Avoid touching the interior surfaces of tubes or plates to prevent introducing impurities. Additionally, keep consumables sealed when not in use to maintain their sterility and integrity.
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